tumor protein 1 wt1  (Boster Bio)

Journal: Frontiers in Oncology

Article Title: Primary extraovarian adult granulosa cell tumor of the greater omentum: a case report and literature review

doi: 10.3389/fonc.2025.1689815

Figure Lengend Snippet: Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) , WT-1 (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).

Article Snippet: WT-1 , Positive , Strong,diffuse nucleus , Supports diagnosis , OTIRIH(Rabbit Monoclonal), Zhongshan Golden Bridge.

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry

Journal: Renal Failure

Article Title: Modified Huangqi Chifeng decoction alleviates podocyte injury on rat with experimental membranous nephropathy

doi: 10.1080/0886022x.2025.2459896

Figure Lengend Snippet: Figure 6. MHCD improved expression of proteins related to podocyte in PHN rats. (A) Immunohistochemistry analysis of nephrin and podocin. Scale bar: 100 µm. (B) Immunohistochemistry analysis of WT-1. Scale bar: 100 µm. (C-D) Representative Western blotting bands and analysis of nephrin and podocin. Each bar represents the mean ± SD of 3 rats for each group. One-way ANOVA followed by tukey’s test. **p < 0.01 versus the normal group. #p < 0.05, ##p < 0.01 versus the model group. &&p < 0.01 versus the benazepril group.

Article Snippet: After antigen retrieval and blocking, immunostaining was performed using the following primary antibodies: Wilms’ tumor protein 1 (WT-1) (No. NBP2-44606, Novus, St. Charles, MO, USA), nephrin (ab216341, abcam, Cambridge, UK) and podocin (ab181143, abcam, Cambridge, UK), followed by HRP-labeled secondary antibody (#31460, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Expressing, Immunohistochemistry, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Characterizing Glomerular Barrier Dysfunction with Patient-Derived Serum in Glomerulus-on-a-Chip Models: Unveiling New Insights into Glomerulonephritis

doi: 10.3390/ijms25105121

Figure Lengend Snippet: Immunofluorescence and Western blot analysis of podocyte-specific markers in undifferentiated and differentiated CIHP-1 cells. Representative immunofluorescence staining for ( A ) podocin (green), ( B ) nephrin (green), ( C ) WT1 (purple), F-actin (red or green), and nuclei (DAPI, blue) in undifferentiated and differentiated CIHP-1 cells. Scale bars: 50 μm. Magnifications: ×200. ( D ) Western blotting of synaptopodin (99 kDa, left panel) and WT1 (55 kDa, right panel) using total protein extracts from undifferentiated and differentiated CIHP-1 cells. GAPDH (37 kDa) was used as a loading control.

Article Snippet: Subsequently, cells were washed with PBS and incubated with primary antibodies for podocin (1:200, Bioss antibodies, Woburn, MA, USA, #bs-6597R), nephrin (1:50, Bioss antibodies, Woburn, MA, USA, #bs-10233R-FITC), and Wilms’ tumor 1 (WT1) (1:100, Bioss antibodies, Woburn, MA, USA, #bs-6983R-A647) diluted in 1% BSA in the dark overnight on a rocker at RT.

Techniques: Immunofluorescence, Western Blot, Staining, Control

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Detailed description of the antibodies used for the tested diagnostic markers.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Diagnostic Assay, Marker, Membrane, Wilms Tumor Assay

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Detailed description of the tested parameters for iView, OptiView, and Envision FLEX detection systems for each of the tested diagnostic markers. Parameters that are bolded were selected as the most optimal among other tested conditions.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Diagnostic Assay, Marker, Incubation, Amplification

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Detailed descriptions of the types of cytological samples used for cytospin preparation and immunocytochemical staining, and the corresponding diagnoses of the patients.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Staining, Marker

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Immunocytochemical staining results for calretinin, Ber-EP4, CK AE1/AE3, CK 5/6, CD68, LCA, desmin, and WT1 with OptiView detection system using different test protocols (×400 magnification). The conditions of each test protocol are labeled directly under the corresponding image within the figure.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Staining, Labeling

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Immunocytochemical staining results for calretinin, Ber-EP4, CK AE1/AE3, CK 5/6, CD68, LCA, desmin, and WT1 with EnVision FLEX (EnVision) detection system using different test protocols (×400 magnification). The conditions of each test protocol are labeled directly under the corresponding image within the figure.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Staining, Labeling

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Comparison of the NEQAS scores, staining results, staining intensity, background, counterstain, and morphology, evaluated on 10 positive cases stained with the optimal OptiView and EnVision protocols and the existing iView protocol for calretinin, Ber-EP4, MOC-31, CK AE1/AE3, CK 5/6, CD68, LCA, desmin, HBME-1, and WT1.

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Comparison, Staining

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: p -values for the comparison of all evaluated parameters described in Table 4 .

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Comparison, Staining

Journal: Diagnostics

Article Title: Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice

doi: 10.3390/diagnostics14060657

Figure Lengend Snippet: Comparison of the selected OptiView and EnVision FLEX immunocytochemical staining protocols for calretinin, Ber-EP4, CK AE1/AE3, CK 5/6, CD68, LCA, desmin, and WT1 with the existing iView protocol (×400 magnification).

Article Snippet: Wilms’ tumor protein 1 (WT1) , BenchMark ULTRA, Dako Omnis , 6F-H2 , Cell Marque, Dako , nucleus , epithelial cells, including normal and malignant cells.

Techniques: Comparison, Staining